Polarized triplet-minus-singlet absorbance difference spectra measured by absorbance-detected magnetic resonance. An application to photosynthetic reaction centres

  • den Blanken H
  • Meiburg R
  • Hoff A
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A method is presented to measure linear dichroic triplet-minus-singlet absorbance difference spectra using absorbance-detected magnetic resonance of the triplet state in zero magnetic field. Orientational selection is achieved by using a linearly-polarised microwave field. The microwave-induced change in the absorbance of light with the electric vector parallel or perpendicular to the microwave field vector is monitored using a Morvue photoelastic modulator and an analyzing polarizer. The method is applied to reaction centres of the photosynthetic bacterium Rhodopseudomonas viridis. © 1984.

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  • H. J. den Blanken

  • R. F. Meiburg

  • A. J. Hoff

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