Currently, information is lacking on gene fl ow in common wheat (Triticum aestivum L.) at dis- tances greater than 300 m based on commer- cial-scale fi elds. The objective of this research was to measure pollen-mediated gene fl ow rates from a blue-aleuroned pollinator (T. aestivum cv. ‘Purendo-38’) to neighboring commercial fi elds of common wheat grown within a 10-km radius of a central pollinator fi eld. In the 2-yr study, 33- ha (2002) and 20-ha (2003) fi elds of Purendo-38 were sown 200 km east-northeast of Saskatoon, Saskatchewan. Sixty-nine fi elds in 2002 and 76 fi elds in 2003 were identifi ed as having overlap- ping fl owering relative to Purendo-38. At matu- rity, up to 2 m2 samples were harvested from each corner of each recipient fi eld. Gene fl ow was identifi ed by the expression of a light-blue pigment in the aleurone layer of F1 hybrid seed. In 2002 one case of gene fl ow was confi rmed at 190 m northeast of the pollinator at a rate of 0.01%. In 2003 nine putative hybrid seeds were confi rmed to be the result of gene fl ow between Purendo-38 and the recipient fi eld using glia- din fi ngerprinting. Consequently, gene fl ow was confi rmed at 0.01% at 500 m northeast, 630 m southeast, and 2.75 km northwest from the pol- linator. In commercial production, gene fl ow in wheat occurs at trace levels (≤ 0.01%) at dis- tances up to 2.75 km.
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