A study, which prepared porous chitosan materials by controlled rate freezing and lyophilization of chitosan solutions and gels and analyzed the physical properties and pore morphology of these materials, suggests that chitosan has good potential as a structural material for assembly of engineered tissues. Chitosan prepared from chitin by deacetylation was carboxymethylated in deferent procedures. It was indicated that the active site of asparaginase would be masked by carboxymethyl-(CM) chitosans in modification and that asparaginase could only bind a certain amount of CM-chitosans.
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