Positional candidate cloning of a QTL in dairy cattle: Identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition

  • Grisart B
  • Coppieters W
  • Farnir F
 et al. 
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We recently mapped a quantitative trait locus (QTL) with a major effect on milk composition—particularly fat content—to the centromeric end of bovine chromosome 14. We subsequently exploited linkage disequilibrium to refine the map position of this QTL to a 3-cM chromosome interval bounded by microsatellite markers BULGE13and BULGE09. We herein report the positional candidate cloning of this QTL, involving (1) the construction of a BAC contig spanning the corresponding marker interval, (2) the demonstration that a very strong candidate gene, acylCoA:diacylglycerol acyltransferase (DGAT1), maps to that contig, and (3) the identification of a nonconservative K232A substitution in the DGAT1 gene with a major effect on milk fat content and other milk characteristics.[The sequence data described in this paper have been submitted to the GenBank data library under accession number AY065621.]

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  • Bernard Grisart

  • Wouter Coppieters

  • Frédéric Farnir

  • Latifa Karim

  • Christine Ford

  • Paulette Berzi

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