Journal article

Potential pathogenic bacteria in metalworking fluids and aerosols from a machining facility

Perkins S, Angenent L ...see all

FEMS Microbiology Ecology, vol. 74, issue 3 (2010) pp. 643-654

  • 17

    Readers

    Mendeley users who have this article in their library.
  • 15

    Citations

    Citations of this article.
  • N/A

    Views

    ScienceDirect users who have downloaded this article.
Sign in to save reference

Abstract

The metalworking and machining industry utilizes recirculating metalworking fluids for integral aspects of the fabrication process. Despite the use of biocides, these fluids sustain substantial biological growth. Subsequently, the high-shear forces incurred during metalworking processing aerosolize bacterial cells and may cause dermatologic and respiratory effects in exposed workers. We quantified and identified the bacterial load for metalworking fluid and aerosol samples of a machining facility in the US Midwest during two seasons. To investigate the presence of potentially pathogenic bacteria in fluid and air, we performed 16S rRNA gene surveys. The concentration of total bacterial cells (including culturable and nonculturable cells) was relatively constant throughout the study, averaging 5.1 × 10⁸ cells mL⁻¹ in the fluids and 4.8 × 10⁵ cells m⁻³ in the aerosols. We observed bacteria of potential epidemiologic significance from several different bacterial phyla in both fluids and aerosols. Most notably, Alcaligenes faecalis was identified through both direct sequencing and culturing in every sample collected. Elucidating the bacterial community with gene surveys showed that metalworking fluids were the source of the aerosolized bacteria in this facility.

Author-supplied keywords

  • 16S rRNA gene survey
  • Alcaligenes faecalis
  • Bacterial load
  • Bioaerosols
  • Metalworking fluids
  • Mycobacterium immunogenum

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • Sarah D. Perkins

  • Largus T. Angenent

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free