Potentiality of the cox1 gene in the taxonomic resolution of soil fungi

  • Molitor C
  • Inthavong B
  • Sage L
 et al. 
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Abstract

We explored the potential of the cox1 gene in the species resolution of soil fungi and compared it with the nuclear internal transcribed spacer (ITS) and small subunit (SSU)-rDNA. Conserved primers allowing the amplification of the fungal cox1 gene were designed, and a total of 47 isolates of Zygomycota and Ascomycota were investigated. The analysis revealed a lack of introns in >90% of the isolates. Comparison of the species of each of the six studied genera showed high interspecific sequence polymorphisms. Indeed, the average of nucleotide variations (4.2-11%) according to the genus, due mainly to the nucleotide substitutions, led to the taxonomic resolution of all the species studied regarding both ITS and SSU-rDNA, in which

Author-supplied keywords

  • Cox1 gene
  • Phylogeny
  • Soil fungi
  • Species resolution

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