Precision cancer mouse models through genome editing with CRISPR-Cas9

  • Mou H
  • Kennedy Z
  • Anderson D
 et al. 
  • 158


    Mendeley users who have this article in their library.
  • 35


    Citations of this article.


The cancer genome is highly complex, with hundreds of point mutations, translocations, and chromosome gains and losses per tumor. To understand the effects of these alterations, precise models are needed. Traditional approaches to the construction of mouse models are time-consuming and laborious, requiring manipulation of embryonic stem cells and multiple steps. The recent development of the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system, a powerful genome-editing tool for efficient and precise genome engineering in cultured mammalian cells and animals, is transforming mouse- model generation. Here, we review how CRISPR-Cas9 has been used to create germline and somatic mouse models with point mutations, deletions and complex chromosomal rearrangements. We highlight the progress and challenges of such approaches, and how these models can be used to understand the evolution and progression of individual tumors and identify new strategies for cancer treatment. The generation of precision cancer mouse models through genome editing will provide a rapid avenue for functional cancer genomics and pave the way for precision cancer medicine. T

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Haiwei Mou

  • Zachary Kennedy

  • Daniel G. Anderson

  • Hao Yin

  • Wen Xue

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free