Preparation and characterization of a pseudo-template imprinted polymer with a chirality-matching monomer for the separation of cinchona alkaloids by high-performance liquid chromatography

Abstract

A hydroquinidine-imprinted stationary selector with chiral recognition for cinchona alkaloids was prepared by bulk polymerization with (+)-(2R,3R)-N, N′-diallyl-L-tartardiamide as a functional monomer, and the recognition properties of the polymers for quinidine (QD) were analyzed with high-performance liquid chromatography. By optimizing the preparation conditions and chromatographic conditions, such as the ratio of the mobile phase and flow rate, QD and quinine could be completely separated to the best resolution of 2.25. The chiral molecularly imprinted polymers (CMIPs) combined the virtues of traditional brush-type chiral selectors with existing CMIPs. The results show a substantial synergistic effect between the chiral monomer and the chiral cavity of the resulting CMIPs in chiral recognition. Copyright © 2013 Wiley Periodicals, Inc.