A Primer on Diagnostic Virology : Direct and Molecular-Based Detection of Viral Pathogens * ABSTRACT : DIRECT DETECTION

  • Yeary T
  • Ascp S
  • Kapil S
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Abstract

T his article describes immunologic and molecular methods that are most com-monly used at noncommercial veterinary diagnostic laboratories. Each approach has advantages in certain circumstances. Determin-ing the best test to request ultimately depends on the case history and proper submission of appropriate specimens. When in doubt, practi-tioners should consult with a diagnostician at their local or regional veterinary diagnostic lab-oratory (see the box on page 739). DIRECT DETECTION A variety of approaches can be used for direct detection of viruses: cell culture (virus isola-tion), electron microscopy, fluorescent antibody (FA) testing, immunohistochemistry, ELISA, and nucleic acid testing. Virus isolation and electron microscopy can be used to identify the presence of an infectious virus or intact viral particles. FA

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  • ISSN: 01931903
  • SCOPUS: 2-s2.0-6344228455
  • PUI: 39393042
  • SGR: 6344228455

Authors

  • Teresa J Yeary

  • S V Ascp

  • Sanjay Kapil

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