Summary Germ-line chimeras provide a valuable approach to surrogate propagation in poultry farming and aquaculture. The appropriate combinations of donor germ cells and host individuals enable the production of eggs and sperm and of offspring from strains with a high commercial value or valuable genotype via an easily reared species or strain. Primordial germ cells (PGCs) are one of the candidate cell types that can be used in the production of germ-line chimeras. The model teleost species, zebrafish, has been used to study specification, differentiation and migration of PGCs. However, it is important that the properties of PGCs are also determined for fish species used in aquaculture. Recent technical developments together with improvements in embryo manipulation techniques have enabled analyses of the differentiation of PGCs and their migration to the genital ridge. Here, we report the characteristic properties of PGCs from several teleost species and investigate their behavior in germ-line chimeras using fluorescently-tagged PGCs.
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