Principles and applications of ligation mediated PCR methods for DNA-based typing of microbial organisms

  • Krawczyk B
  • Kur J
  • Stojowska-Swedrzyńska K
 et al. 
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Outbreaks of infectious disease often result from exposure to a common source of the etiologic agent. Generally, the etiologic agent causing an outbreak of infection is derived from a single cell whose progeny are genetically identical or closely related to the source organism. In pidemiological terms, the organisms involved in the outbreak are clonally related; that is, they have a common origin. Clonally related organisms are members of the same species that share virulence factors, biochemical traits, and genomic characteristics. However, there is sufficient diversity at the species level that organisms isolated from different sources at different times and in different geographical regions

Author-supplied keywords

  • Genotyping
  • Microbial phylogenetics
  • Molecular epidemiology
  • PCR (polymerase chain reaction)
  • Rapid methods

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