Production of microbial oil with high oleic acid content by Trichosporon capitatum

  • Wu H
  • Li Y
  • Chen L
 et al. 
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Abstract

Microbial oils with high unsaturated fatty acids content, especially oleic acid content, are good feedstock for high quality biodiesel production. Trichosporon capitatum was found to accumulate lipid with around 80% oleic acid and 89% total unsaturated fatty acids content on nitrogen-limited medium. In order to improve its lipid yield, effects of medium components and culture conditions on cell growth and lipid accumulation were investigated. Optimization of media resulted in a 61% increase in the lipid yield of T. capitatum after cultivation at 28°C and 160. rpm for 6. days. In addition, T. capitatum could grow well on cane molasses and afford a lipid yield comparable to that on synthetic nitrogen-limited medium. The biodiesel from the microbial oil produced by T. capitatum on cane molasses displayed a low cold filter plugging point (-15°C), and so T. capitatum might be a promising strain to provide lipid suitable for high quality biodiesel production. © 2010 Elsevier Ltd.

Author-supplied keywords

  • Acid content
  • Biodiesel
  • Biodiesel production
  • Cane molasses
  • Cell culture
  • Cold filter plugging point
  • Culture conditions
  • Dipodascus capitatus
  • Growth kinetics
  • High oleic acid content
  • High quality
  • High unsaturated fatty acid
  • Medium components
  • Microbial oil
  • Molasses
  • Oleic acid
  • Trichosporon
  • Trichosporon capitatum
  • Unsaturated fatty acids
  • bioaccumulation
  • biofuel
  • cultivation
  • enzyme activity
  • fatty acid
  • fungus
  • optimization
  • qualitative analysis

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Authors

  • H Wu

  • Y Li

  • L Chen

  • M Zong

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