Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines

  • Wang P
  • Mariman E
  • Keijer J
 et al. 
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Abstract

Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation was screened, and 41 proteins that responded to blocking of secretion by 20degreesC treatment and/or brefeldin A treatment were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25 are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an actively remodeling extracellular matrix and several factors involved in growth regulation.

Author-supplied keywords

  • 3T3-L1 adipocyte
  • Extracellular matrix
  • Growth regulation
  • Proteomics
  • Secretion

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