Pronounced effect of DNA hybridization on click reaction efficiency

  • Wirges C
  • Gramlich P
  • Gutsmiedl K
 et al. 
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Abstract

We report about the efficiency of the click reaction directly on PCR products containing alkyne-modified uridine nucleosides. Galactose azide was clicked onto single-stranded and double-stranded 300mer DNA in order to investigate the efficiency of sugar labeling of DNA under various conditions. Single-stranded alkyne-modified DNA was prepared by selective lambda exonuclease digestion of 5prime-phosphate-labeled double strands. A comparison of the click reaction efficiency on modified single and double stranded 300mers under identical conditions shows a pronounced effect of the strandedness on the yield of the click reaction.

Author-supplied keywords

  • Click reaction
  • DNA labeling efficiency
  • Lambda exonuclease digest

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Authors

  • Christian T. Wirges

  • Philipp M.E. Gramlich

  • Katrin Gutsmiedl

  • Johannes Gierlich

  • Glenn A. Burley

  • Thomas Carell

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