Protein engineering of Acidovorax facilis 72W nitrilase for bioprocess development

  • Wu S
  • Fogiel A
  • Petrillo K
 et al. 
  • 23


    Mendeley users who have this article in their library.
  • 24


    Citations of this article.


Hydroxycarboxylic acid monomers can be used to prepare industrially important polymers. Enzymatic production of such hydroxycarboxylic acids is often preferred to chemical production since the reactions are run at ambient temperature, do not require strongly acidic or basic reaction conditions, and produce the desired product with high selectivity at high conversion. However, native enzymes often do not perform desired reactions with the efficiency required for commercial applications. Protein engineering was used to significantly increase the specific activity of nitrilase from Acidovorax facilis 72W for the conversion of 3-hydroxyvaleronitrile to 3-hydroxyvaleric acid. Overexpression of engineered nitrilase enzymes in Escherichia coli, combined with immobilization of whole cells in alginate beads that can be recycled many times has facilitated the development of a commercially viable bioprocess for production of 3-hydroxyvaleric acid.

Author-supplied keywords

  • 3-hydroxyvaleric acid
  • Bioprocess development
  • Nitrilase
  • Protein engineering

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Shijun Wu

  • Arthur J. Fogiel

  • Kelly L. Petrillo

  • Eugenia C. Hann

  • Lawrence J. Mersinger

  • Robert DiCosimo

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free