This review provides an overview on the methods available for analysis of O-glycosylation. Three major themes are addressed: analysis of released O-glycans including different O-glycan liberation, derivatization, and detection methods; analysis of formerly O-glycosylated peptides yielding information on O-glycan attachment sites; analysis of O-glycopeptides, representing by far the most informative but also most challenging approach for O-glycan analysis. Although there are various techniques available for the identification of O-linked oligosaccharides, the focus here is on MS fragmentation techniques such as collision-induced fragmentation, electron capture dissociation, and electron transfer dissociation. Finally, the O-glycan analytical challenges that need to be met will be discussed. Copyright © by Walter de Gruyter • Berlin • Boston.
CITATION STYLE
Zauner, G., Kozak, R. P., Gardner, R. A., Fernandes, D. L., Deelder, A. M., & Wuhrer, M. (2012, August). Protein O-glycosylation analysis. Biological Chemistry. https://doi.org/10.1515/hsz-2012-0144
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