Proteomic profiling of cerebrospinal fluid identifies biomarkers for amyotrophic lateral sclerosis

  • Ranganathan S
  • Williams E
  • Ganchev P
 et al. 
  • 66

    Readers

    Mendeley users who have this article in their library.
  • 156

    Citations

    Citations of this article.

Abstract

Amyotrophic lateral sclerosis (ALS) is characterized by degeneration of motor neurons. We tested the hypothesis that proteomic analysis will identify protein biomarkers that provide insight into disease pathogenesis and are diagnostically useful. To identify ALS specific biomarkers, we compared the proteomic profile of cerebrospinal fluid (CSF) from ALS and control subjects using surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF-MS). We identified 30 mass ion peaks with statistically significant (p < 0.01) differences between control and ALS subjects. Initial analysis with a rule-learning algorithm yielded biomarker panels with diagnostic predictive value as subsequently assessed using an independent set of coded test subjects. Three biomarkers were identified that are either decreased (transthyretin, cystatin C) or increased (carboxy-terminal fragment of neuroendocrine protein 7B2) in ALS CSF. We validated the SELDI-TOF-MS results for transthyretin and cystatin C by immunoblot and immunohistochemistry using commercially available antibodies. These findings identify a panel of CSF protein biomarkers for ALS.

Author-supplied keywords

  • Amyotrophic lateral sclerosis
  • Cerebrospinal fluid
  • Mass spectrometry
  • Proteomics

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Eric WilliamsCornell University

    Follow
  • Srikanth Ranganathan

  • Philip Ganchev

  • Vanathi Gopalakrishnan

  • David Lacomis

  • Leo Urbinelli

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free