Purification of synthetic peptides using a catching full-length sequence by polymerization approach

  • Zhang M
  • Pokharel D
  • Fang S
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Abstract

During automated solid-phase peptide synthesis, failure sequences were capped with acetic anhydride. After synthesis, a polymerizable methacrylamide tag was attached to the full-length sequences. Peptide purification was then achieved by polymerizing the full-length sequences, washing away impurities, and cleaving the peptide product from the polymer.

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