Pyrosequencing for the rapid detection of rifampicin resistance in Mycobacterium tuberculosis: a meta-analysis.

  • Guo Q
  • Zheng R
  • Zhu C
 et al. 
  • 17

    Readers

    Mendeley users who have this article in their library.
  • N/A

    Citations

    Citations of this article.

Abstract

BACKGROUND: Multidrug-resistant tuberculosis is a major threat to the control of tuberculosis and to public health. Whereas most conventional methods of drug susceptibility testing (DST) are precise but time consuming, pyrosequencing is a rapid, high-throughput technique.

OBJECTIVE: To conduct a meta-analysis to evaluate the overall accuracy of pyrosequencing for the detection of rifampicin (RMP) resistance.

METHODS: We searched PubMed, Web of Science, Elsevier and BIOSIS databases according to a written protocol and explicit study selection criteria. A summary receiver operating characteristic curve (SROC) and Cochrane (Q*) index were calculated to perform this meta-analysis using Meta-Disc software.

RESULTS: Twelve studies involving 594 specimens with RMP resistance and 793 RMP-susceptible specimens met the inclusion criteria. Of these, 11 were based on Mycobacterium tuberculosis clinical isolates. The overall sensitivity and specificity were estimated at respectively 0.94 (95%CI 0.92-0.96) and 0.98 (95%CI 0.97-0.99). The area under the SROC curve was 0.99 and the Cochrane (Q*) index was 0.96. For clinical specimens, the overall sensitivity and specificity estimates were respectively 0.89 (range 0.52-1.00) and 0.99 (range 0.95-1.00).

CONCLUSIONS: This meta-analysis shows that pyrosequencing is a highly sensitive and specific tool for the detection of RMP resistance in M. tuberculosis. The pyrosequencing assay is conducted in a high-throughput format, with a turnaround time of

Author-supplied keywords

  • Antibiotics, Antitubercular
  • Antibiotics, Antitubercular: pharmacology
  • Drug Resistance, Bacterial
  • High-Throughput Screening Assays
  • High-Throughput Screening Assays: methods
  • Humans
  • Microbial Sensitivity Tests
  • Microbial Sensitivity Tests: methods
  • Mycobacterium tuberculosis
  • Mycobacterium tuberculosis: drug effects
  • Rifampin
  • Rifampin: pharmacology
  • Sensitivity and Specificity
  • Time Factors

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Q Guo

  • R-J Zheng

  • C-T Zhu

  • L-L Zou

  • J-F Xiu

  • J Li

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free