Quantification of CFTR transcripts.

  • Ramalho A
  • Clarke L
  • Amaral M
  • 12


    Mendeley users who have this article in their library.
  • 6


    Citations of this article.


Quantification and analysis of CFTR transcripts is of crucial importance not only for cystic fibrosis (CF) diagnosis and prognosis, but also in evaluating the efficiency of various therapeutic approaches to CF, including gene therapy. Reverse transcription (RT) followed by quantitative polymerase chain reaction (qPCR) is at present the most sensitive method for transcript abundance measurement. Classical RNA-based methods require significant expression levels in target samples for appropriate analysis, thus PCR-based methods have evolved towards reliable quantification. In this chapter we describe and discuss several protocols for the quantitative analysis of CFTR transcripts, including those variants that result from alternative splicing.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text


  • Anabela S. Ramalho

  • Luka A. Clarke

  • Margarida D. Amaral

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free