Quantitation of Dyes in Forensic Fibers Using UHPLC-TOF MS and Building a Dye Database with AxION EC ID Software

  • Spectrometry M
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Abstract

Fibers originating from textiles in carpet, furniture, and clothing are often used as evidence at crime scenes. Fiber analysis is traditionally performed using various non-destructive techniques including optical microscopy, visible and infrared micro-spectrophotometry. Chromatography techniques such as poor resolving thin-layer chromatography or higher resolution HPLC with UV or diode array detection (DAD) can also be used for fiber identification. In this study, we show analysis of dyes extracted from fibers using UHPLC coupled to time-of-flight mass spectrometry (TOF MS), providing a more specific and sensitive technique. TOF MS provides a higher degree of discrimination between closely resembling dyes with similar functional groups of detection compared to UV or DAD detection using accurate mass measurements and isotope ratio confirmations. We also provide a method to build a database for forensic dyes using accurate mass and isotope profile information acquired from the time-of-flight (TOF) mass spectrometer along with the proprietary AxION® EC ID™ software.

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APA

Spectrometry, M. (2016). Quantitation of Dyes in Forensic Fibers Using UHPLC-TOF MS and Building a Dye Database with AxION EC ID Software. UPLC-TOF-MS.

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