Quantitation of latent varicella-zoster virus and herpes simplex virus genomes in human trigeminal ganglia

  • Pevenstein S
  • Williams R
  • McChesney D
 et al. 
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Abstract

Using real-time fluorescence PCR, we quantitated the numbers of copies of latent varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) genomes in 15 human trigeminal ganglia. Eight (53%) and 1 (7%) of 15 ganglia were PCR positive for HSV-1 or -2 glycoprotein G genes, with means of 2,902 +/- 1,082 (standard error of the mean) or 109 genomes/10(5) cells, respectively. Eleven of 14 (79%) to 13 of 15 (87%) of the ganglia were PCR positive for VZV gene 29, 31, or 62. Pooling of the results for the three VZV genes yielded a mean of 258 +/- 38 genomes/10(5) ganglion cells. These levels of latent viral genome loads have implications for virus distribution in and reactivation from human sensory ganglia.

Author-supplied keywords

  • *Genome, Viral
  • *Virus Latency
  • Adolescent
  • Adult
  • Aged
  • Chickenpox/pathology/virology
  • DNA, Viral
  • Female
  • Herpes Genitalis/pathology/virology
  • Herpes Simplex/pathology/virology
  • Herpesvirus 1, Human/genetics/*isolation & purific
  • Herpesvirus 2, Human/genetics/*isolation & purific
  • Herpesvirus 3, Human/genetics/*isolation & purific
  • Humans
  • Immediate-Early Proteins/genetics
  • Male
  • Middle Aged
  • Polymerase Chain Reaction/methods
  • Trans-Activators/genetics
  • Trigeminal Ganglion/*virology
  • Viral Envelope Proteins/genetics
  • Viral Load

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Authors

  • S R Pevenstein

  • R K Williams

  • D McChesney

  • E K Mont

  • J E Smialek

  • S E Straus

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