Quantitative Determination of Trypsin Inhibitory Activity in Complex Matrices

  • E.J. Spelbrink R
N/ACitations
Citations of this article
57Readers
Mendeley users who have this article in their library.

Abstract

A quantitative assay using azocasein was developed to measure trypsin inhibitory activity in emulsions and other complex systems that are refractory to analysis. The method was tested for reproducibility on pure protein solutions as well as protein-containing material rich in fats and sugars, with special attention to emulsions. In the clean situation, the overall relative standard deviation was less than 6% while for the more complex systems it was less than 16%. The proce-dure proved robust against deliberate variations of temperature, incubation time and substrate concentration.

Cite

CITATION STYLE

APA

E.J. Spelbrink, R. (2011). Quantitative Determination of Trypsin Inhibitory Activity in Complex Matrices. The Open Food Science Journal, 5(1), 42–46. https://doi.org/10.2174/1874256401105010042

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free