Quantitative Optimization of Reverse Transfection Conditions for 384-Well siRNA Library Screening

  • LaPan P
  • Zhang J
  • Pan J
 et al. 
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A necessary step in all small interfering RNA (siRNA) library screens is introduction of the siRNA into cells. We describe the use of a commercially available glyceraldehyde 3-phosphate dehydrogenase enzymatic assay that is capable of simultaneously assessing the efficiency of siRNA delivery into cells and the lipid toxicity. This assay has been modified to work in 384-well plates using reverse transfection. The assay is fast, inexpensive, and quantitative. Conditions identified as optimal using this technique have been employed successfully in library screens.

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  • Peter LaPan

  • Jing Zhang

  • Jing Pan

  • Steve Haney

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