Quantitative Optimization of Reverse Transfection Conditions for 384-Well siRNA Library Screening

  • LaPan P
  • Zhang J
  • Pan J
 et al. 
  • 10

    Readers

    Mendeley users who have this article in their library.
  • 4

    Citations

    Citations of this article.

Abstract

A necessary step in all small interfering RNA (siRNA) library screens is introduction of the siRNA into cells. We describe the use of a commercially available glyceraldehyde 3-phosphate dehydrogenase enzymatic assay that is capable of simultaneously assessing the efficiency of siRNA delivery into cells and the lipid toxicity. This assay has been modified to work in 384-well plates using reverse transfection. The assay is fast, inexpensive, and quantitative. Conditions identified as optimal using this technique have been employed successfully in library screens.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Peter LaPan

  • Jing Zhang

  • Jing Pan

  • Steve Haney

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free