A necessary step in all small interfering RNA (siRNA) library screens is introduction of the siRNA into cells. We describe the use of a commercially available glyceraldehyde 3-phosphate dehydrogenase enzymatic assay that is capable of simultaneously assessing the efficiency of siRNA delivery into cells and the lipid toxicity. This assay has been modified to work in 384-well plates using reverse transfection. The assay is fast, inexpensive, and quantitative. Conditions identified as optimal using this technique have been employed successfully in library screens. © Mary Ann Liebert, Inc. 2008.
CITATION STYLE
LaPan, P., Zhang, J., Pan, J., & Haney, S. (2008). Quantitative optimization of reverse transfection conditions for 384-well siRNA library screening. Assay and Drug Development Technologies, 6(5), 683–691. https://doi.org/10.1089/adt.2008.142
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