A quantitative PCR assay for the detection and quantification of Shiga toxin-producing Escherichia coli (STEC) in minced beef and dairy products

  • Derzelle S
  • Grine A
  • Madic J
 et al. 
  • 37

    Readers

    Mendeley users who have this article in their library.
  • 19

    Citations

    Citations of this article.

Abstract

Shiga toxin (Stx)-producing Escherichia coli (STEC) are amongst major causes of food-borne infectious diseases and outbreaks. A new quantitative PCR (qPCR) assay was designed to detect all known stx gene subtypes in a single reaction, including the most distant variant stx2f. Performance of this assay was evaluated in combination with two different internal amplification controls (IAC), a competitive one specific for the assay and a universal IAC based on plasmid pUC19. The qPCR assay was 100% specific and showed analytical sensitivity of two STEC genome copies per reaction. The diagnostic approach proposed, combining enrichment, automated DNA extraction and qPCR detection, could reliably detect the presence of STEC in minced beef and cheese inoculated before enrichment at

Author-supplied keywords

  • Escherichia coli
  • Food analysis
  • QPCR
  • STEC
  • Shiga toxin
  • Stx

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • S. Derzelle

  • A. Grine

  • J. Madic

  • C. Peytavin de Garam

  • N. Vingadassalon

  • F. Dilasser

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free