A quantitative PCR assay for the detection and quantification of Shiga toxin-producing Escherichia coli (STEC) in minced beef and dairy products

  • Derzelle S
  • Grine A
  • Madic J
 et al. 
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Shiga toxin (Stx)-producing Escherichia coli (STEC) are amongst major causes of food-borne infectious diseases and outbreaks. A new quantitative PCR (qPCR) assay was designed to detect all known stx gene subtypes in a single reaction, including the most distant variant stx2f. Performance of this assay was evaluated in combination with two different internal amplification controls (IAC), a competitive one specific for the assay and a universal IAC based on plasmid pUC19. The qPCR assay was 100% specific and showed analytical sensitivity of two STEC genome copies per reaction. The diagnostic approach proposed, combining enrichment, automated DNA extraction and qPCR detection, could reliably detect the presence of STEC in minced beef and cheese inoculated before enrichment at

Author-supplied keywords

  • Escherichia coli
  • Food analysis
  • QPCR
  • STEC
  • Shiga toxin
  • Stx

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  • S. Derzelle

  • A. Grine

  • J. Madic

  • C. Peytavin de Garam

  • N. Vingadassalon

  • F. Dilasser

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