Quantitative study of doxorubicin in living cell nuclei by microspectrofluorometry

  • Gigli M
  • Doglia S
  • Millot J
 et al. 
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Abstract

Doxorubicin-DNA association has been studied by quantitative microspectrofluorometry. Fluorescence emission spectra from a microvolume of single living cell nuclei treated with doxorubicin have been analyzed in terms of difference in spectral shape and fluorescence yield between free and DNA-bound drug. Contribution of each spectral component to the total signal was calculated by least-squares linear regression. With this method of analysis, total drug concentration has been determined with an error of less than 10%. Moreover, the uptake into the nucleus has been studied in a non destructive way, avoiding use of14C-labelled drug. Kinetic studies of drug accumulation into the nuclei were conducted on sensitive and resistant cells. © 1988.

Author-supplied keywords

  • (Intact nucleus)
  • DNA-drug interaction
  • Doxorubicin
  • Drug uptake
  • Microspectrofluorometry

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Authors

  • Mauro Gigli

  • Silvia M. Doglia

  • Jean M. Millot

  • Luigi Valentini

  • Michel Manfait

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