Quantitative super-resolution imaging of Bruchpilot distinguishes active zone states

  • Ehmann N
  • Van De Linde S
  • Alon A
 et al. 
  • 165

    Readers

    Mendeley users who have this article in their library.
  • 65

    Citations

    Citations of this article.

Abstract

The precise molecular architecture of synaptic active zones (AZs) gives rise to different structural and functional AZ states that fundamentally shape chemical neurotransmission. However, elucidating the nanoscopic protein arrangement at AZs is impeded by the diffraction-limited resolution of conventional light microscopy. Here we introduce new approaches to quantify endogenous protein organization at single-molecule resolution in situ with super-resolution imaging by direct stochastic optical reconstruction microscopy (dSTORM). Focusing on the Drosophila neuromuscular junction (NMJ), we find that the AZ cytomatrix (CAZ) is composed of units containing ~137 Bruchpilot (Brp) proteins, three quarters of which are organized into about 15 heptameric clusters. We test for a quantitative relationship between CAZ ultrastructure and neurotransmitter release properties by engaging Drosophila mutants and electrophysiology. Our results indicate that the precise nanoscopic organization of Brp distinguishes different physiological AZ states and link functional diversification to a heretofore unrecognized neuronal gradient of the CAZ ultrastructure.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Nadine Ehmann

  • Sebastian Van De Linde

  • Amit Alon

  • Dmitrij Ljaschenko

  • Xi Zhen Keung

  • Thorge Holm

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free