Laser-excited Raman spectra of rRNA from Escherichia coli have been obtained in the frequency range 2000-200 cm-1for both H2O and2H2O solutions. Raman lines of the phosphodiester group and of paired and unpaired purine and pyrimidine bases are readily detected. Several lines in the spectra are sensitive to changes in RNA secondary structure caused by increasing the ionic strength of solutions. The results suggest that the Raman effect will be more advantageous than infrared spectroscopy for the study of secondary structures and intermolecular interactions of nucleic acids in aqueous solution. © 1970.
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