Raman spectroscopy for the non-contact and non-destructive monitoring of collagen damage within tissues

  • Votteler M
  • Carvajal Berrio D
  • Pudlas M
 et al. 
  • 45


    Mendeley users who have this article in their library.
  • 39


    Citations of this article.


The non-destructive and label-free monitoring of extracellular matrix (ECM) remodeling and degradation processes is a great challenge. Raman spectroscopy is a non-contact method that offers the possibility to analyze ECM in situ without the need for tissue processing. Here, we employed Raman spectroscopy for the detection of heart valve ECM, focusing on collagen fibers. We screened the leaflets of porcine aortic valves either directly after dissection or after treatment with collagenase. By comparing the fingerprint region of the Raman spectra of control and treated tissues (400-1800 cm(-1)), we detected no significant differences based on Raman shifts; however, we found that increasing collagen degradation translated into decreasing Raman signal intensities. After these proof-of-principal experiments, we compared Raman spectra of native and cryopreserved valve tissues and revealed that the signal intensities of the frozen samples were significantly lower compared to those of native tissues, similar to the data seen in the enzymatically-degraded tissues. In conclusion, our data demonstrate that Raman microscopy is a promising, non-destructive and non-contact tool to probe ECM state in situ.

Author-supplied keywords

  • Collagen
  • Cryopreservation
  • Extracellular matrix
  • Heart valves
  • Raman spectroscopy

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Miriam Votteler

  • Daniel A. Carvajal Berrio

  • Marieke Pudlas

  • Heike Walles

  • Ulrich A. Stock

  • Katja Schenke-Layland

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free