Rapid DNA library construction for functional genomic and metagenomic screening.

  • Schmitz J
  • Daniel A
  • Collin M
 et al. 
  • 2

    Readers

    Mendeley users who have this article in their library.
  • N/A

    Citations

    Citations of this article.

Abstract

A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.

Author-supplied keywords

  • Bacterial Toxins
  • Bacterial Toxins: genetics
  • Cloning
  • Escherichia coli
  • Gene Library
  • Genetic
  • Genetic Testing
  • Genetic Testing: methods
  • Genomics
  • Genomics: methods
  • Molecular
  • Nucleic Acid Amplification Techniques
  • Plasmids
  • Plasmids: genetics
  • Pore Forming Cytotoxic Proteins
  • Pore Forming Cytotoxic Proteins: genetics
  • Transcription

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Jonathan E Schmitz

  • Anu Daniel

  • Mattias Collin

  • Vincent a Fischetti

  • Metagenomic Screening

  • Raymond Schuch

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free