Rapid DNA Library Construction for Functional Genomic and Metagenomic Screening

  • Schmitz J
  • Daniel A
  • Collin M
 et al. 
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Abstract

A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample. 10.1128/AEM.01864-07

Author-supplied keywords

  • genomics
  • metagenomics

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Authors

  • Jonathan Schmitz

  • Anu Daniel

  • Mattias Collin

  • Raymond Schuch

  • Vincent Fischetti

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