Rapid DNA library construction for functional genomic and metagenomic screening.

  • Schmitz J
  • Daniel A
  • Collin M
 et al. 
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A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.

Author-supplied keywords

  • Bacterial Toxins
  • Bacterial Toxins: genetics
  • Cloning
  • Escherichia coli
  • Gene Library
  • Genetic
  • Genetic Testing
  • Genetic Testing: methods
  • Genomics
  • Genomics: methods
  • Molecular
  • Nucleic Acid Amplification Techniques
  • Plasmids
  • Plasmids: genetics
  • Pore Forming Cytotoxic Proteins
  • Pore Forming Cytotoxic Proteins: genetics
  • Transcription

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  • Jonathan E Schmitz

  • Anu Daniel

  • Mattias Collin

  • Vincent a Fischetti

  • Metagenomic Screening

  • Raymond Schuch

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