Abstract
A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
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CITATION STYLE
Schmitz, J. E., Daniel, A., Collin, M., Schuch, R., & Fischetti, V. A. (2008). Rapid DNA library construction for functional genomic and metagenomic screening. Applied and Environmental Microbiology, 74(5), 1649–1652. https://doi.org/10.1128/AEM.01864-07
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