PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis. Copyright © 2014, American Society for Microbiology. All Rights Reserved.
CITATION STYLE
Chang, Y. T., Wang, H. C., Wang, M. C., Wu, A. B., Sung, J. M., Sun, H. S., … Wu, C. J. (2014). Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry. Journal of Clinical Microbiology, 52(4), 1217–1219. https://doi.org/10.1128/JCM.03106-13
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