Rapid Identification of Biothreat and Other Clinically Relevant Bacterial Species by Use of Universal PCR Coupled with High-Resolution Melting Analysis Rapid Identification of Biothreat and Other Clinically Relevant Bacterial Species by Use of Universal P

  • Yang S
  • Ramachandran P
  • Rothman R
 et al. 
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Abstract

A rapid assay for eubacterial species identification is described using high-resolution melt analysis to characterize PCR products. Unique melt profiles generated from multiple hypervariable regions of the 16S rRNA gene for 100 clinically relevant bacterial pathogens, including category A and B biothreat agents and their surrogates, allowed highly specific species identification.

Author-supplied keywords

  • 16S
  • 16S: genetics
  • Bacteria
  • Bacteria: isolation & purification
  • Bacterial
  • Bacterial Infections
  • Bacterial Infections: diagnosis
  • Bacterial: genetics
  • Bioterrorism
  • DNA
  • Environmental Microbiology
  • Humans
  • Polymerase Chain Reaction
  • Polymerase Chain Reaction: methods
  • RNA
  • Ribosomal
  • Ribosomal: genetics
  • Transition Temperature

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Authors

  • Samuel Yang

  • Padmini Ramachandran

  • Richard Rothman

  • Yu-hsiang Hsieh

  • Andrew Hardick

  • Helen Won

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