A rapid method to attain isotope labeled small soluble peptides for NMR studies

  • Koenig B
  • Rogowski M
  • Louis J
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Abstract

A widely applicable strategy is presented for efficient and rapid production of small water soluble peptides expressed as fusion proteins with the immunoglobulin-binding domain of streptococcal protein G. A simple extraction and purification scheme that includes a protease cleavage step to release the target peptide is described. The yield of authentic target peptide exceeds 10 mg per liter of culture. Production of U-13C, 15N and highly deuterated U-13C, 15N isotope labeled peptide is demonstrated for the 11 residue S2 peptide, corresponding to the C-terminus of the alpha-subunit of transducin, and the coiled coil trimerization domain from cartilage matrix protein (CMPcc), respectively. Heteronuclear two-dimensional NMR spectra are used for initial peptide characterization.

Author-supplied keywords

  • E. coli
  • Fusion protein
  • Isotope labeling
  • NMR
  • Recombinant peptide

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Authors

  • Bernd W. Koenig

  • Marco Rogowski

  • John M. Louis

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