Rapid and specific efflux of reduced glutathione during apoptosis induced by anti-Fas/APO-1 antibody

  • Van den Dobbelsteen D
  • Nobel C
  • Schlegel J
 et al. 
  • 28


    Mendeley users who have this article in their library.
  • 325


    Citations of this article.


Although human JURKAT T lymphocytes induced to undergo apoptosis with anti-Fas/APO-1 antibody were observed to rapidly lose reduced glutathione (GSH), increased concentrations of oxidized products were not detectable. Unexpectedly, the reduced tripeptide was instead quantitatively recovered in the incubation medium of the cells. As GSH loss was blocked by bromosulfophthalein and dibromosulfophthalein, known inhibitors of hepatocyte GSH transport, a specific export rather than nonspecific leakiness through plasma membranes is proposed to be responsible. Apoptosis was delayed when GSH-diethylesters were used to elevate intracellular GSH, although the high capacity of the activated efflux system quickly negated the benefit of this treatment. Stimulation of GSH efflux provides a novel mechanism whereby Fas/APO-1 ligation can deplete GSH. We speculate that it enhances the oxidative tonus of a responding cell without requiring an increase in the production of reactive oxygen species.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Diels J. Van den Dobbelsteen

  • C. Stefan I Nobel

  • Jörg Schlegel

  • Ian A. Cotgreave

  • Sten Orrenius

  • Andrew F G Slater

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free