Ras signalling on the endoplasmic reticulum and the Golgi

  • Chiu V
  • Bivona T
  • Hach A
 et al. 
  • 168


    Mendeley users who have this article in their library.
  • 444


    Citations of this article.


Current models evoke the plasma membrane (PM) as the exclusive platform from which Ras regulates signalling. We developed a fluorescent probe that reports where and when Ras is activated in living cells. We show that oncogenic H-Ras and N-Ras engage Raf-1 on the Golgi and that endogenous Ras and unpalmitoylated H-Ras are activated in response to mitogens on the Golgi and endoplasmic reticulum (ER), respectively. We also demonstrate that H-Ras that is restricted to the ER can activate the Erk pathway and transform fibroblasts, and that Ras localized on different membrane compartments differentially engages various signalling pathways. Thus, Ras signalling is not limited to the PM, but also proceeds on the endomembrane.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Vi K. Chiu

  • Trever Bivona

  • Angela Hach

  • J. Bernard Sajous

  • Joseph Silletti

  • Heidi Wiener

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free