Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange

  • Ding Y
  • Li J
  • Enterina J
 et al. 
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Abstract

We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca(2+) concentration dynamics and other second-messenger signaling activities.

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Authors

  • Yidan Ding

  • Jiao Li

  • Jhon Ralph Enterina

  • Yi Shen

  • Issan Zhang

  • Paul H. Tewson

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