Ratiometric Imaging of pH Probes

  • Grillo-Hill B
  • Webb B
  • Barber D
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Abstract

Measurement of intracellular pH can be readily accomplished using tools and methods described in this chapter. We present a discussion of technical considerations of various ratiometric pH-sensitive probes including dyes and genetically encoded sensors. These probes can be used to measure pH across physical scales from macroscopic whole-mount tissues down to organelles and subcellular domains. We describe protocols for loading pH-sensitive probes into single cells or tissues and discuss ratiometric image acquisition and analysis. © 2014 Elsevier Inc.

Author-supplied keywords

  • Drosophila melanogaster
  • Genetically encoded pH biosensor
  • In situ imaging
  • Live-cell imaging
  • PH measurement
  • PH-sensitive dyes
  • Quantitative imaging
  • Ratiometric imaging
  • Whole-mount tissue imaging

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