Real-Time Nucleic Acid Sequence-Based Amplification in Nanoliter Volumes

  • Gulliksen A
  • Solli L
  • Karlsen F
 et al. 
  • 44


    Mendeley users who have this article in their library.
  • 81


    Citations of this article.


Real-time nucleic acid sequence-based amplification (NASBA) is an isothermal method specifically designed for amplification of RNA. Fluorescent molecular beacon probes enable real-time monitoring of the amplification process. Successful identification, utilizing the real-time NASBA technology, was performed on a microchip with oligonucleotides at a concentration of 1.0 and 0.1 microM, in 10- and 50-nL reaction chambers, respectively. The microchip was developed in a silicon-glass structure. An instrument providing thermal control and an optical detection system was built for amplification readout. Experimental results demonstrate distinct amplification processes. Miniaturized real-time NASBA in microchips makes high-throughput diagnostics of bacteria, viruses, and cancer markers possible, at reduced cost and without contamination.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Anja Gulliksen

  • Lars Solli

  • Frank Karlsen

  • Henrik Rogne

  • Eivind Hovig

  • Trine Nordstrøm

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free