Two perchlorate-reducing bacterial consortia (PRBC) were obtained by enrichment cultures from polluted marine sediments. Non-salt-tolerant PRBC (N-PRBC) was enriched without the addition of NaCl, and salt tolerant-PRBC (ST-PRBC) was enriched with 30g-NaClL-1. Although the perchlorate reduction rates decreased with increasing NaCl concentration, ST-PRBC (resp., N-PRBC) could reduce perchlorate until 75g-NaClL-1(resp., 30g-NaClL-1). The reduction yield (1.34±0.05mg-perchlorate per mg-acetate) and maximum perchlorate reduction rate (86mg-perchlorateL-1h-1) of ST-PRBC was higher than those (1.16±0.03mg-perchlorate per mg-acetate and 48mg-perchlorateL-1h-1) of N-PRBC. Kinetic analysis showed that NaCl acted as an uncompetitive inhibitor against both PRBCs. The inhibition constants were 25 and 41mg-NaClL-1for N-PRBC and ST-PRBC, respectively. © 2011 Elsevier Ltd.
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