Regenerative Surface Plasmon Resonance (SPR) biosensor: Real-time measurement of fibrinogen in undiluted human serum using the competitive adsorption of proteins

  • Wang R
  • Lajevardi-Khosh A
  • Choi S
 et al. 
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Abstract

Epidemiological studies suggest that elevated plasma fibrinogen levels are associated with an increased risk of cardiovascular disorders. Normal fibrinogen level is in the range of 1.5-4.5. mg/mL, depending upon both genetic (intrinsic) and environmental (extrinsic) factors. An increase of 0.25 mg/mL from the normal level can often be correlated with a high risk of cardiovascular disease. Thus, it is useful to monitor fibrinogen level in serum of a patient for clinical diagnosis. We report a regenerative biosensor that measures real-time fibrinogen levels in undiluted serum. The biosensor uses Surface Plasmon Resonance (SPR), highly sensitive optical technique. The biosensor does not use bio-receptors (i.e., antibodies, enzymes, DNA, etc.) unlike conventional biosensors, and deploys the nature of competitive adsorption of proteins to achieve selective detection of fibrinogen. We measured fibrinogen-spiked serum samples with a concentration of 1.5-4.5. mg/mL, and repeated six measurement trials to obtain statistical distribution of the measurements using the regeneration method of the sensing surface. The SPR biosensor has a sensitivity of 42 mDeg/(mg/mL) for a fibrinogen concentration in the range of 0.5-2.5. mg/mL, whereas it was hard to correlate the measurements to the spiked-fibrinogen samples of above 2.5. mg/mL. © 2011 Elsevier B.V.

Author-supplied keywords

  • Fibrinogen
  • Human serum
  • Regenerative biosensor
  • Surface Plasmon Resonance (SPR)
  • Vroman effect

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Authors

  • R. Wang

  • A. Lajevardi-Khosh

  • S. Choi

  • J. Chae

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