The LasR-LasI and RhlR-RhlI quorum-sensing systems are global regulators of gene expression in the opportunistic pathogen Pseudomonas aeruginosa. Previous studies suggest that the RhlR-RhlI system activates expression of rpoS. We constructed merodiploid strains of P. aeruginosa containing the native rpoS gene and an rpoS-lacZ fusion. Studies of lacZ transcription in these strains indicated that rpoS was not regulated by RhlR-RhlI. We also generated an rpoS null mutant. This rpoS mutant showed elevated levels of rhll (but not rhlR) transcription, elevated levels of the RhlI-generated acylhomoserine lactone quorum- sensing signal, and elevated levels of RhlR-RhlI-regulated gene transcription. These findings indicate that there is a relationship between RpoS and quorum sensing, but rather than the RhlR-RhlI system influencing the expression of rpoS, it appears that RpoS regulates rhlL.
CITATION STYLE
Whiteley, M., Parsek, M. R., & Greenberg, E. P. (2000). Regulation of quorum sensing by RpoS in Pseudomonas aeruginosa. Journal of Bacteriology, 182(15), 4356–4360. https://doi.org/10.1128/JB.182.15.4356-4360.2000
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