Relative effect of transcription-level and translation-level control of protein synthesis during early development of the sea urchin.

  • Terman S
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Abstract

Newly synthesized proteins were pulse labeled with radioactive amino acids at several developmental stages of the sea urchin, Arbacia punctulata-in normal embryos and in embryos continuously exposed to the drug Dactinomycin. The soluble proteins were fractionated by electrophoresis through polyacrylamide gels. A simple procedure for complete solubilization of the proteins in slices of gels into a solution of toluene for liquid scintillation counting, and a computer program to display and to compare statistically the distributions of (14)C- and (3)H-labeled proteins fractionated on the same gel, allowed quantitative statements to be made on the relative changes in protein synthesis. The conclusion is that translation-level control provides most of the changes in protein synthesis that occur between fertilization and hatched blastula stage.

Author-supplied keywords

  • Animals
  • Carbon Isotopes
  • Dactinomycin
  • Dactinomycin: pharmacology
  • Disc
  • Echinodermata
  • Electrophoresis
  • Embryo
  • Genetic
  • Genetic Code
  • Leucine
  • Leucine: metabolism
  • Messenger
  • Messenger: metabolism
  • Nonmammalian
  • Nonmammalian: drug effects
  • Nonmammalian: metabolism
  • Protein Biosynthesis
  • Proteins
  • Proteins: analysis
  • RNA
  • Templates
  • Tritium

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Authors

  • S A Terman

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