Microwave-assisted ammonium hydroxide (NH4OH) followed by phosphoric acid (H3PO4) treatments were used to release monomeric sugars from Miscanthus sinensis grown in Cha-Chueng-Sao province, Thailand. Treatment with 1.0% (w/v) NH4OH, 15:1 liquid-to-solid ratio (LSR) at 120°C temperature for 15min liberated 2.9g of monomeric sugars per 100g of dried biomass, whereas the corresponding yield for a treatment with 1.78% v/v H3PO4, 15:1 LSR at 140°C for 30min was 62.3g/100g. The two-stage pretreatment, treatment with NH4OH at 120°C temperature for 15min followed by treatment with H3PO4at 140°C for 30min, impressively provided the highest total monomeric sugar yield of 71.6g/100g dried biomass. © 2011 Elsevier Ltd.
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