A reliable method for individual identification and gender determination of wild leopards (Panthera pardus fusca) using non-invasive samples

  • Dutta T
  • Sharma S
  • Maldonado J
 et al. 
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Abstract

We describe a highly polymorphic microsatellite panel for identifying individual leopards using DNA from scat. After successfully screening 16 published microsatellites, we optimized a panel of 7 microsatellites that yields a Probability of Identity between siblings value of 5.24E-04. We used this panel to identify 217 individuals from 287 leopard scats collected from five tiger-reserves in Central India. We identified 101 males and 92 females by amplifying a fragment of the Amelogenin protein gene. This panel will be helpful to study genetic structure, gene flow, relatedness and sex ratio of leopards.

Author-supplied keywords

  • Individual identification
  • Microsatellite
  • Noninvasive DNA sampling
  • Panthera pardus fusca
  • Sex assignment

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