Remodelling of actin cytoskeleton in tomato cells in response to inoculation with a biocontrol strain of Fusarium oxysporum in comparison to a pathogenic strain

  • Humbert C
  • Aimé S
  • Alabouvette C
 et al. 
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Accepted Article Tel: 33 (0)3 80 69 30 50 Fax: 33 (0)3 80 69 32 24 Keywords: TRITC-phalloidin, fluorescence microscopy, cytochalasins, tomato cell suspension, cell death. Running head: Actin cytoskeleton response to F. oxysporum ABSTRACT Knowing that actin microfilaments play a key role in the mobilization of the defence-associated cellular responses, the aim of this study was to compare changes affecting the actin cytoskeleton in tomato cells after inoculation with germinated microconidia of a bio-control (Fo47, Fom24) or a pathogenic (Fol8) strain of F. oxysporum. Actin microfilaments were observed by labelling with TRITC-phalloidin combined with fluorescence microscopy. Results showed that only tenuous changes in the actin cytoskeleton architecture occurred after inoculation with the bio-control strains whereas the actin cytoskeleton was significantly altered after inoculation with the pathogenic one. In the two types of interaction, cell death occurs and can be considered as one key component of cell defence responses. A pharmacological approach using cytochalasins was chosen to determine if the inhibition of actin polymerization differently affects the kinetics of tomato cell death. Data showed that cytochalasins reduced cell death induced by the bio-control strain Fo47, and in contrast, increased cell death induced by the pathogenic strain Fol8, suggesting that the pathway leading to cell death differs in the protective and compatible interactions. INTRODUCTION

Author-supplied keywords

  • Cell death
  • Cytochalasins
  • Fluorescence microscopy
  • TRITC-phalloidin
  • Tomato cell suspension

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