Removal of deaminated cytosines and detection of in vivo methylation in ancient DNA

  • Briggs A
  • Stenzel U
  • Meyer M
 et al. 
  • 250

    Readers

    Mendeley users who have this article in their library.
  • 84

    Citations

    Citations of this article.

Abstract

DNA sequences determined from ancient organisms have high error rates, primarily due to uracil bases created by cytosine deamination. We use synthetic oligonucleotides, as well as DNA extracted from mammoth and Neandertal remains, to show that treatment with uracil-DNA-glycosylase and endonuclease VIII removes uracil residues from ancient DNA and repairs most of the resulting abasic sites, leaving undamaged parts of the DNA fragments intact. Neandertal DNA sequences determined with this protocol have greatly increased accuracy. In addition, our results demonstrate that Neandertal DNA retains in vivo patterns of CpG methylation, potentially allowing future studies of gene inactivation and imprinting in ancient organisms.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Adrian W. Briggs

  • Udo Stenzel

  • Matthias Meyer

  • Johannes Krause

  • Martin Kircher

  • Svante Pääbo

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free