Repair of potentially lethal damage does not depend on functional TP53 in human glioblastoma cells.

  • van Bree C
  • Franken N
  • Rodermond H
 et al. 
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Abstract

The functionality of G(1)-phase arrest was investigated in relation to repair of potentially lethal damage (PLD) in human glioblastoma Gli-06 cells. Confluent cultures were irradiated and plated for clonogenic survival either immediately or 24 h after gamma irradiation. Bivariate flow cytometry was performed to assess the distribution over the cell cycle. Levels of TP53 and CDKN1A protein were assessed with Western blotting and levels of CDKN1A mRNA with RT-PCR. Confluence significantly reduced the number of proliferating cells. Marked PLD repair was found in the absence of an intact G(1) arrest. No accumulation of TP53 was observed, and the protein was smaller than the wild-type TP53 of RKO cells. No increased expression of CDKN1A at the mRNA or protein levels was found in Gli-06 cells. The TP53 of Gli-06 cells was unable to transactivate the CDKN1A gene. From this study, it is evident that PLD repair may be present without a functional TP53 or G(1) arrest.

Author-supplied keywords

  • Apoptosis
  • Apoptosis: radiation effects
  • Cell Division
  • Cell Division: radiation effects
  • Cell Line, Tumor
  • DNA
  • DNA Damage
  • DNA Repair
  • DNA Repair: radiation effects
  • DNA: radiation effects
  • Dose-Response Relationship, Radiation
  • Glioblastoma
  • Glioblastoma: metabolism
  • Glioblastoma: pathology
  • Humans
  • Radiation Dosage
  • Tumor Suppressor Protein p53
  • Tumor Suppressor Protein p53: metabolism

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Authors

  • C van Bree

  • N a P Franken

  • H M Rodermond

  • L J a Stalpers

  • J Haveman

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