Research on extraction and characterization of cellulase from commercial enzyme preparation

  • Shu G
  • Yang H
  • Chen H
 et al. 
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Abstract

The extraction of cellulase from commercial enzyme preparation by ammonium sulfate precipitation and its enzymatic characterization were studied. The results were as follows: the conditions of precipitation was 60% saturation of ammonium sulfate, the recovery rate of carboxymethylcellulase (CMCase) and Filter Paper enzyme Activity (FPA) were 73.8 and 71.4%, respectively. The enzyme has optimal temperature of 55°C, optimal pH of 4.8, the ions of Co2+, Mn2+ in the buffer lowered the activity of CMCase, but the Cu2+ in low concentration activated the CMCase. The ions of Co2+, Mn2+ and Al3+ in the buffer lowered the activity of FPA, but the Cu2+and K+ in low concentration activated the FPA; Km and Vmax of the enzyme were 2.22×10-3g/mL and 1.11×104 U/h using Lineweaver-Burk graphic method, respectively. © Maxwell Scientific Organization, 2013.

Author-supplied keywords

  • Carboxymethylcellulase
  • Cellulase
  • Enzymatic property
  • Filter paper activity
  • Salting out

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  • ISSN: 20424868
  • PUI: 369463551
  • SCOPUS: 2-s2.0-84880942043
  • SGR: 84880942043

Authors

  • Guowei Shu

  • Hui Yang

  • He Chen

  • Zhiwei Yang

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